Review



ice cold 1× chip sonication nuclear lysis buffer  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cell Signaling Technology Inc ice cold 1× chip sonication nuclear lysis buffer
    Ice Cold 1× Chip Sonication Nuclear Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 14484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/Cell+Lysis+Buffer/pm40344061-231-15-23
    Average 99 stars, based on 14484 article reviews
    ice cold 1× chip sonication nuclear lysis buffer - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. The pellet was resuspended in 1 ml of ice-cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500-μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15-s on and 30-s off cycles for 25 min. After sonication, lysates were combined into a 1.5-ml Eppendorf tube and clarified by centrifugation at 21,100 g for 10 min at 4°C.

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. 11, eadu8014 (2025) 9 May 2025 10 of 13 was resuspended in 1 ml of ice- cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500- μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15- s on and 30- s off cycles for 25 min. After sonication, lysates were combined into a 1.5- ml Eppendorf tube and clarified by centrifugation at 21,100g for 10 min at 4°C.

    Sonication:

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. The pellet was resuspended in 1 ml of ice-cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500-μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15-s on and 30-s off cycles for 25 min. After sonication, lysates were combined into a 1.5-ml Eppendorf tube and clarified by centrifugation at 21,100 g for 10 min at 4°C.

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. 11, eadu8014 (2025) 9 May 2025 10 of 13 was resuspended in 1 ml of ice- cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500- μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15- s on and 30- s off cycles for 25 min. After sonication, lysates were combined into a 1.5- ml Eppendorf tube and clarified by centrifugation at 21,100g for 10 min at 4°C.

    Lysis:

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. The pellet was resuspended in 1 ml of ice-cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500-μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15-s on and 30-s off cycles for 25 min. After sonication, lysates were combined into a 1.5-ml Eppendorf tube and clarified by centrifugation at 21,100 g for 10 min at 4°C.

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. 11, eadu8014 (2025) 9 May 2025 10 of 13 was resuspended in 1 ml of ice- cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500- μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15- s on and 30- s off cycles for 25 min. After sonication, lysates were combined into a 1.5- ml Eppendorf tube and clarified by centrifugation at 21,100g for 10 min at 4°C.

    Protease Inhibitor:

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. Cell pellet was resuspended in 1 ml of 1× ChIP Sonication Cell Lysis Buffer (Cell Signaling Technology, 96529) with Protease Inhibitor Cocktail (PIC; Cell Signaling Technology, 7012) and stored at −80°C if not processed immediately. ..

    Polymerase Chain Reaction:

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST
    Article Snippet: .. The pellet was resuspended in 1 ml of ice-cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500-μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15-s on and 30-s off cycles for 25 min. After sonication, lysates were combined into a 1.5-ml Eppendorf tube and clarified by centrifugation at 21,100 g for 10 min at 4°C.

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. 11, eadu8014 (2025) 9 May 2025 10 of 13 was resuspended in 1 ml of ice- cold 1× ChIP Sonication Nuclear Lysis Buffer (Cell Signaling Technology, 28778) with PIC and aliquoted into four 500- μl PCR tubes for sonication. .. Lysates were sonicated at 70% amplitude with 15- s on and 30- s off cycles for 25 min. After sonication, lysates were combined into a 1.5- ml Eppendorf tube and clarified by centrifugation at 21,100g for 10 min at 4°C.



    Similar Products

    99
    Thermo Fisher chip sonication buffer
    Chip Sonication Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/HEPES/pmc12993448-105-5-46
    Average 99 stars, based on 1 article reviews
    chip sonication buffer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc ice cold 1× chip sonication nuclear lysis buffer
    Ice Cold 1× Chip Sonication Nuclear Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/Cell+Lysis+Buffer/pm40344061-231-15-23
    Average 99 stars, based on 1 article reviews
    ice cold 1× chip sonication nuclear lysis buffer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc chip sonication cell lysis buffer
    Chip Sonication Cell Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/Cell+Lysis+Buffer/pm40344061-225-9-14
    Average 99 stars, based on 1 article reviews
    chip sonication cell lysis buffer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc chip sonication lysis buffer
    Chip Sonication Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/chip+lysis+buffer/pmc11725848__pnas__2422321121__sapp-64-4-11
    Average 90 stars, based on 1 article reviews
    chip sonication lysis buffer - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc chip sonication nuclear lysis buffer
    Chip Sonication Nuclear Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/SimpleChIP+Sonication+Cell+and+Nuclear+Lysis+Buffers/pmc10810857-118-29-34
    Average 93 stars, based on 1 article reviews
    chip sonication nuclear lysis buffer - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc 2x chip sonication cell lysis buffer
    2x Chip Sonication Cell Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/Cell+Lysis+Buffer/pmc10810857-118-23-34
    Average 99 stars, based on 1 article reviews
    2x chip sonication cell lysis buffer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    90
    Cosmo Bio USA chip sonication nuclear lysis buffer
    Chip Sonication Nuclear Lysis Buffer, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+sonication+buffer/chip+sonication+nuclear+lysis+buffer/pm38268203-38-10-25
    Average 90 stars, based on 1 article reviews
    chip sonication nuclear lysis buffer - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    Image Search Results